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Clearing a Potential Road Block to Bisabolane

By Guest PostJanuary 14, 2012Scienceabies grandis, abies grandis α bisabolene synthase, advanced biofuels, agbis, andy degiovanni, berkeley lab, biofuel, biosynthetic alternative, bisabolane, ca, chemical compounds, cofactors, d2 diesel fuel, emeryville, enzyme, evolutionary heritage, flavorings, fragrances, grand fir, green energy, inhibitors, jay keasling, jose pereira, masood hadi, microbes, microbial based production, pamela peralta yahya, paul adams, precursor, protein, protein crystal structure, renewable alternative, resolution, ryan mcandrew, synthases in plants, terpene class, terpenoid synthases, three dimensional crystal structure, trucking industry, united states, x ray crystallography

*Joint BioEnergy Institute Researchers Identify Key Enzyme Structure*

The recent discovery that bisabolane, a member of the terpene class of chemical compounds used in fragrances and flavorings, holds high promise as a biosynthetic alternative to D2 diesel fuel has generated keen interest in the green energy community and the trucking industry. Now a second team of researchers with the U.S Department of Energy (DOE)’s Joint BioEnergy Institute (JBEI) has determined the three-dimensional crystal structure of a protein that is key to boosting the microbial-based production of bisabolane as an advanced biofuel.

The JBEI research team, led by bioengineers Paul Adams and Jay Keasling, solved the protein crystal structure of an enzyme in the Grand fir (Abies grandis) that synthesizes bisabolene, the immediate terpene precursor to bisabolane. The performance of this enzyme – the Abies grandis α-bisabolene synthase (AgBIS) – when engineered into microbes, has resulted in a bottleneck that hampers the conversion by the microbes of simple sugars into bisabolene. (more…)

E. Coli Bacteria Engineered to Eat Switchgrass and Make Transportation Fuels

By Guest PostDecember 4, 2011Environment, Scienceagricultural waste, anthe george, bacteria, berkeley lab, biofuel production, biofuels, blake simmons, bradley holmes, cellulolytic, cellulose, chemistry, christopher voigt, danielle tullman ercek, diesel, e coli, enzyme, eric steen, gasoline, green, gregory bokinsky, hemicellulolytic, hemicelluloses, ionic liquid, jay keasling, jbei, jeffrey dietrich, jet fuels, joint bioenergy institute, lignin, lignocellulosic biomass, lignocellulosic feedstocks, microbes, milestone, molten salt, pamela peralta yahya, renewable alternative, strains of escherichia coli bacteria, sugars, switchgrass biomass, synthesize, taek soon lee, woody material

*Joint BioEnergy Institute (JBEI) Researchers Reach Milestone on the Road to Biofuels*

A milestone has been reached on the road to developing advanced biofuels that can replace gasoline, diesel and jet fuels with a domestically-produced clean, green, renewable alternative.

Researchers with the U.S. Department of Energy (DOE)’s Joint BioEnergy Institute (JBEI) have engineered the first strains of Escherichia coli bacteria that can digest switchgrass biomass and synthesize its sugars into all three of those transportation fuels. What’s more, the microbes are able to do this without any help from enzyme additives. (more…)

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Clearing a Potential Road Block to Bisabolane

E. Coli Bacteria Engineered to Eat Switchgrass and Make Transportation Fuels